{"id":2112,"date":"2026-02-23T14:52:20","date_gmt":"2026-02-23T14:52:20","guid":{"rendered":"https:\/\/chromosome.ie\/?page_id=2112"},"modified":"2026-04-10T22:42:56","modified_gmt":"2026-04-10T21:42:56","slug":"mirkovic","status":"publish","type":"page","link":"https:\/\/chromosome.ie\/gae\/groups\/mirkovic\/","title":{"rendered":"Mihailo Mirkovic"},"content":{"rendered":"<div class=\"wp-block-image is-style-default\">\n<figure class=\"alignleft size-thumbnail\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/02\/Mihailo-150x150.jpg\" alt=\"\" class=\"wp-image-2132\" style=\"aspect-ratio:1;object-fit:cover\" srcset=\"https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/02\/Mihailo-150x150.jpg 150w, https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/02\/Mihailo-300x300.jpg 300w, https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/02\/Mihailo-12x12.jpg 12w, https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/02\/Mihailo.jpg 461w\" sizes=\"auto, (max-width: 150px) 100vw, 150px\" \/><\/figure>\n<\/div>\n\n\n<p><\/p>\n\n\n\n<p><strong>Dr. Mihailo Mirkovic<\/strong><br>Senior Lecturer<br>mihailo.mirkovic@universityofgalway.ie<br>Welcome Trust Career Development Award<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\"\/>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">\u00c1bhair Sp\u00e9ise<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Mitosis<\/li>\n\n\n\n<li>Chromosome Instability<\/li>\n\n\n\n<li>Introns<\/li>\n\n\n\n<li>Aneuploidy<\/li>\n\n\n\n<li>Adaptation through aneuploidy<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<h4 class=\"wp-block-heading\">Achoimre Taighde<\/h4>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<p class=\"has-text-align-justify\">We study how fungi exploit Chromosome Instability (CIN) to adapt to stress.&nbsp; Mistakes during mitosis result in CIN and unbalanced, aneuploid karyotypes. In most contexts, aneuploidy lowers cell fitness. However, in fungi, CIN and aneuploidy can be beneficial during stress. Aneuploid karyotypes can survive diverse stress conditions, from elevated temperatures to antifungal treatment, pointing to CIN as a driver of adaptation. Intriguingly, the rate of CIN is elevated in stress, opening the possibility that cells have pathways to destabilize their genomes and  accelerate adaptation.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"462\" height=\"534\" src=\"https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/03\/Mihailo-Fig1.png\" alt=\"\" class=\"wp-image-2148\" srcset=\"https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/03\/Mihailo-Fig1.png 462w, https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/03\/Mihailo-Fig1-260x300.png 260w, https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/03\/Mihailo-Fig1-10x12.png 10w\" sizes=\"auto, (max-width: 462px) 100vw, 462px\" \/><figcaption class=\"wp-element-caption\"><em>An example of Chromosome Instability (CIN) in budding yeast. Chromosome II is directly visualized using a Chr II Tet-O array and expression of TetR-GFP that binds to the array. In normal mitosis (top), chromosomes are partitioned symmetrically between the mother and daughter cell. In the case of chromosome instability (CIN), both copies (marked by a clustered, single, brighter foci) end up either in the mother or the daughter cell, generating unbalanced, aneuploid karyotypes (Adapted from <a href=\"https:\/\/elifesciences.org\/reviewed-preprints\/104530\">Mirkovic et al 2025<\/a>).<\/em><\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p class=\"has-text-align-justify\">Using <em>Saccharomyces cerevisiae<\/em> agus <em>Candida albicans<\/em>, we will tackle the following questions:<\/p>\n\n\n\n<p class=\"has-text-align-justify\"><em><mark style=\"background-color:#f2f2f2\" class=\"has-inline-color\">How do cells sense stress and how does this translate into CIN?<\/mark><\/em><\/p>\n\n\n\n<p class=\"has-text-align-justify\">Stress results in drastic alteration of pre-mRNA is processing, affecting the function of intron-containing genes. Our previous work has shown that altered pre-mRNA processing and the presence of introns contribute to CIN in ageing. We will explore the role of pre-mRNA processing and introns as drivers of CIN in stress.<\/p>\n\n\n\n<p><em><mark style=\"background-color:#f2f2f2\" class=\"has-inline-color has-black-color\">Which features of the genome drive elevated CIN?<\/mark><\/em><\/p>\n\n\n\n<p class=\"has-text-align-justify\">While the plasticity of fungal genomes during stress is well documented, the drivers are poorly understood. Using the power of genome-wide screens in fungi, we will discover the drivers of CIN in different stress conditions.<\/p>\n\n\n\n<p><em><mark style=\"background-color:#f2f2f2\" class=\"has-inline-color\">Is instability a feature, rather than a bug?<\/mark><\/em><\/p>\n\n\n\n<p class=\"has-text-align-justify\">Discovering pathways responsible for CIN in stress allows us to directly test its role in adaptation, by manipulating CIN rates. If instability is required for adaptation, elevated CIN could be a stress response, rather than a \u201cmistake\u201d.<\/p>\n\n\n\n<div class=\"wp-block-essential-blocks-advanced-image  root-eb-advanced-image-sqsx7\"><div class=\"eb-parent-wrapper eb-parent-eb-advanced-image-sqsx7\"><figure class=\"eb-advanced-image-wrapper eb-advanced-image-sqsx7 img-style-rounded caption-style-2 caption-horizontal-center caption-vertical-bottom bottom no-effect\" data-id=\"eb-advanced-image-sqsx7\"><div class=\"image-wrapper\"><img decoding=\"async\" src=\"https:\/\/chromosome.ie\/wp-content\/uploads\/2026\/03\/Mihaile-Fig2.png\" alt=\"\"\/><\/div><figcaption><em>Elevated CIN in stress could be a selected trait, not an error. Cells which can induce instability have higher karyotype variance in stress, allowing them to generate aneuploid karyotypes which can survive the stress (top). If there is no elevated CIN (bottom), there is less variance, and less potential for adaptation.<\/em><\/figcaption><\/figure><\/div><\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Cro\u00edtheichn\u00edochat\u00ed Taighde<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Micreasc\u00f3ipeacht<\/li>\n\n\n\n<li>Modhn\u00fa G\u00e9an\u00f3im<\/li>\n\n\n\n<li>Yeast genetics<\/li>\n\n\n\n<li>Genome-wide screens<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Eochairfhocail<\/h4>\n\n\n\n<p>Mitosis; Introns; RNA processing; Adaptation; Chromosome Instability; Yeast; Antifungal resistance; Aneuploidy<\/p>\n<\/div>\n<\/div>\n\n\n\n<h4 class=\"wp-block-heading\"><\/h4>\n\n\n\n<p><\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Foilseach\u00e1in Roghnaithe<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/elifesciences.org\/reviewed-preprints\/104530\" target=\"_blank\" rel=\"noreferrer noopener\">Dissociation of the nuclear basket triggers chromosome loss in aging yeast<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/elifesciences.org\/articles\/71196\" target=\"_blank\" rel=\"noreferrer noopener\">DNA circles promote yeast ageing in part through stimulating the reorganization of nuclear pore complexes<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/journals.plos.org\/plosbiology\/article?id=10.1371\/journal.pbio.3000016\" target=\"_blank\" rel=\"noreferrer noopener\">Induced aneuploidy in neural stem cells triggers a delayed stress response and impairs adult life span in flies<\/a> <\/li>\n\n\n\n<li><a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6191932\/\" target=\"_blank\" rel=\"noreferrer noopener\">Absence of the Spindle Assembly Checkpoint Restores Mitotic Fidelity upon Loss of Sister Chromatid Cohesion<\/a> <\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Foilseach\u00e1in is D\u00e9ana\u00ed<\/h4>\n\n\n<ul class=\"wp-block-rss\"><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/pubmed.ncbi.nlm.nih.gov\/41165159\/?utm_source=WordPress&#038;utm_medium=rss&#038;utm_campaign=pubmed-2&#038;utm_content=1VmNOZgUnBp0VQe5nHSOhTPSGSV070SEgcwSrULfQ6GMG_PkMH&#038;fc=20260223101250&#038;ff=20260423041249&#038;v=2.19.0.post6+133c1fe'>Dissociation of the nuclear basket triggers chromosome loss in aging yeast<\/a><\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/pubmed.ncbi.nlm.nih.gov\/35373738\/?utm_source=WordPress&#038;utm_medium=rss&#038;utm_campaign=pubmed-2&#038;utm_content=1VmNOZgUnBp0VQe5nHSOhTPSGSV070SEgcwSrULfQ6GMG_PkMH&#038;fc=20260223101250&#038;ff=20260423041249&#038;v=2.19.0.post6+133c1fe'>DNA circles promote yeast ageing in part through stimulating the reorganization of nuclear pore complexes<\/a><\/div><\/li><li class='wp-block-rss__item'><div class='wp-block-rss__item-title'><a href='https:\/\/pubmed.ncbi.nlm.nih.gov\/30794535\/?utm_source=WordPress&#038;utm_medium=rss&#038;utm_campaign=pubmed-2&#038;utm_content=1VmNOZgUnBp0VQe5nHSOhTPSGSV070SEgcwSrULfQ6GMG_PkMH&#038;fc=20260223101250&#038;ff=20260423041249&#038;v=2.19.0.post6+133c1fe'>Induced aneuploidy in neural stem cells triggers a delayed stress response and impairs adult life span in flies<\/a><\/div><\/li><\/ul>\n\n\n<h4 class=\"wp-block-heading\">Nascanna \u00c1isi\u00fala<\/h4>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p>Pr\u00f3f\u00edl Ollscoile<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-text-align-center\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=%220000-0003-0802-7200%22+OR+%22Mihailo+Mirkovic%22&amp;sort=pubdate\">Pub Med<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-text-align-right\"><a href=\"https:\/\/scholar.google.com\/citations?user=UffkjIkAAAAJ&amp;hl=en\">Google Scholar<\/a><\/p>\n<\/div>\n<\/div>\n\n\n\n<h4 class=\"wp-block-heading\">T\u00e9igh i dTeagmh\u00e1il!<\/h4>\n\n\n\n<p>mihailo.mirkovic@universityofgalway.ie<\/p>\n\n\n\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Dr. Mihailo MirkovicSenior Lecturermihailo.mirkovic@universityofgalway.ieWelcome Trust Career Development Award Research interests Research overview We study how fungi exploit Chromosome Instability (CIN) to adapt to stress.&nbsp; Mistakes during mitosis result in CIN and unbalanced, aneuploid karyotypes. In most contexts, aneuploidy lowers cell&#8230; <a class=\"more-link\" href=\"https:\/\/chromosome.ie\/gae\/groups\/mirkovic\/\">Continue Reading &rarr;<\/a><\/p>","protected":false},"author":3,"featured_media":0,"parent":7,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","_eb_attr":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-2112","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/pages\/2112","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/comments?post=2112"}],"version-history":[{"count":33,"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/pages\/2112\/revisions"}],"predecessor-version":[{"id":2186,"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/pages\/2112\/revisions\/2186"}],"up":[{"embeddable":true,"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/pages\/7"}],"wp:attachment":[{"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/media?parent=2112"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/categories?post=2112"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/chromosome.ie\/gae\/wp-json\/wp\/v2\/tags?post=2112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}